[Cnidaria] whole mount slide preparation protocols

Lisa-ann Gershwin lisa.gershwin at stingeradvisor.com
Sat May 2 21:51:40 PDT 2009


Yeah, Glycergel -- it's magic!

Step 1. Warm Glycergel in a mug of hot water.
Step 2. Put the wet live or formalin-fixed critter on the slide, add 
a drop or two of Glycergel (one if you will be pressing down, two if 
not), cover with coverslip, let dry. Voila.

They will do much better over the long term if preserved, but will 
still look pretty good over the short term if mounted live.

It's made by Dako.

Cheers, Lisa



At 04:01 AM 29/04/2009, you wrote:
>Content-class: urn:content-classes:message
>Content-Type: multipart/alternative;
>         boundary="----_=_NextPart_001_01C9C82B.516019AE"
>
>Hi folks!
>
>I am hoping to create some permanent whole mount slides of ephyrae 
>to serve as training tools for those new to jelly culture and 
>identification.  Does anyone out there have some established and 
>effective techniques or protocols for such an endeavor?  Any tips or 
>suggestions would be greatly appreciated.  Many thanks for your time 
>and consideration.
>
>regards,
>Michael
>
>Michael Howard
>Husbandry Division
>Monterey Bay Aquarium
>_______________________________________________
>List-Info: https://maillists.uci.edu/mailman/listinfo/cnidaria




===============================================================
Dr. Lisa-ann Gershwin
Director, Australian Marine Stinger Advisory Services
Email: lisa.gershwin at stingeradvisor.com.au
Mob: +61 (0)438 105 358

Australian Marine Stinger Advisory Services
ABN 94 698 104 687
Post Office Box 5559
TOWNSVILLE QLD 4810
www.stingeradvisor.com.au 
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