[Cnidaria] whole mount slide preparation protocols

[Julian Smith III]

I made wholemount slides of ephyrae and other plankters  in TEM 
embedding epoxy (stain, dehydrate in EtOH, then clear/infiltrate/embed 
using acetone as an intermediary solvent).  Support coverslips with 
glass tubing pulled fine in a flame, and polymerize in a 60° oven as 
usual.  No problems if the slides are stored on edge.  For fixation, I 
used 4% formaldehyde in seawater and stained in Lynch's precipitated 
borax carmine with fast green counterstain.
HTH
Julian

Michael Howard wrote:
>
> Hi folks!
>
> I am hoping to create some permanent whole mount slides of ephyrae to 
> serve as training tools for those new to jelly culture and 
> identification.  Does anyone out there have some established and 
> effective techniques or protocols for such an endeavor?  Any tips or 
> suggestions would be greatly appreciated.  Many thanks for your time 
> and consideration.
>
> regards,
> Michael
>
>
> Michael Howard
> Husbandry Division
> Monterey Bay Aquarium
>
> ------------------------------------------------------------------------
>
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-- 
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC  29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)