[Cnidaria] identifying cells in Nematostella In-situ experiment.

Dror Hilman dror.hilman at mail.huji.ac.il
Thu Jun 19 01:33:17 PDT 2008


Dear all,
I need some help to identify cells in Nematostella.
I recently performed whole-mount In-situ hybridization of Nematostella
adult (polyp).
The probe for the hybridization was the Nematostella ortholog for
neuronal - elav gene, a RNA binding protein, that used as neuronal
marker in mammals.
The color substrate is NBT-BCIP.
I have got a nice staining of the tentacles and the mouth (attached picture).
After the hybridization, I made cryo-section of the tissue, and I am
not sure about the identity of the cells that were stained.
Can you help me identify the cells - are they neurons?

Dror Hilman,
Hebrew University,
Dept. of cell and animal biology,
Jerusalem, Israel
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